414 A Novel Psoriasis Model

Psoriasis is a chronic inflammatory skin disorder characterized by hyperproliferation and aberrant differentiation of keratinocytes, inflammation in dermis epidermis leukocyte infiltration Th1-like cytokine pattern. An experimental mechanism-based vitro model has been developed using novel multi-compartment reconstructed full-thickness (3C System Episkin SA) applying systemically mixture Th-1 cytokines (IL-17, IL-22 TNFα) at defined concentration (1,5ng/ml each 2 ml medium) for 48h. To better mimick the contribution immune system psoriasis, THP-1 cells were co-cultured responsiveness to treatment was assessed topical application two anti-psoriatic reference drugs: Product A containing mometasone furoate B calcipotriol betametasone. After 48h, with or without 24h recovery period absence mixture, tissues (in triplicate) harvested gene expression PI3 (SKALP), S100A7 (psoriasin 1) SERPINIB4 RT-PCR histomorphological analysis hematoxylin/Eosin Staining. In psoriasis induced series (Positive Control) 3 genes resulted overexpressed H&E staining revealed modified morphology particular spinous granular layers epidermis. At 48h both products have shown positive efficacy counteracting psoriatic transcriptional profile vs PC: decreased while all three validated genes. results same as except SERPINB4 which found basal level PC after B. The new 3C stimulated THP1 on basis PI3, SEPINIB4 This advanced responsive drugs can be used discriminate product investigate their mechanisms action.